Urea biosynthesis in a surviving amphibian liver cube preparation.
نویسندگان
چکیده
The biosynthesis of urea in vifro was maintained at a constant rate for 48 hours in a surviving amphibian liver cube system. The maintenance of constant oxygen consumption and protein synthesis during this time indicated that the majority of cells remained viable. A direct correlation between the rate of lixation of 14C02 and ammonia into urea and the levels of carbamyl phosphate synthetase in vifro was similar to observations made in the metamorphosing amphibian in vivo.
منابع مشابه
The Relation of Oxygen Supply to Water Movement and to Urea Formation in Surviving Liver Tissue
Liver slices have been immersed during periods up to 4 hours at 38 degrees C. in Krebs-Ringer solution with bicarbonate buffer and exposed to varied oxygen supply in the presence of carbon dioxide equal to that of venous blood. Water movement, urea, and amino acid formation by the liver tissue have been measured. Water contents of surviving liver tissue diminishes with increased oxygen supply, ...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 245 16 شماره
صفحات -
تاریخ انتشار 1970